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KMID : 0356919700030010107
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Korean Journal of Anesthesiology
1970 Volume.3 No. 1 p.107 ~ p.112
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Antimycobacterial Activities of Dodecyl diaminothyl glycin hydrochloride as a Disinfectant
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黃淸子/Whang, C.C.
陳在新/金周德/吳興根/Chin, J.S./Kim, J.D./Oh, H.K.
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Abstract
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In order to prevent cross-infection, the sterilization of used and/ or contaminated anesthetic apparatus is still a difficult problem in clinical practice, particularly, following anesthesia for known. Advanced pulmonary tuberculosis cases. Therefore the antibacterial activities of an amphotericdetergent, Dodecyl diaminoethyl glycin hydrochloride (Hygien 51) were tested in vitro as a disinfectant against mycobacteria (M. tuberculosis 1L T Rv, M. bovis, M. avium, M. tuberculosis Hsr R4, M. phlei, B.C.G.)
The antimycobacterial activity tests wereperfomed according to the modified Kolmer method_ The testing organisms were cultured in Dubos broth at 37°C, and the detergent, Hygien 51, was diluted with sterile distilled water to 1, 3, 5 and 10%. One half milliliter of culture was pipetted into each tube containing 4.5 ml of,- diluted detergent, and at intervals of 3, 15, 30,?60, 120, 180´ and 240 minutes, a 4mm loopful of the material was removed from each tube, and transferred to. Ogawa media. The tests were carried out at two different temperatures, 20°C and 50°C The subcultures were incubated at 37°C for more than 8 weeks. The results were obtained as follows:
1) At room temperature (20°C), all mycobateria, except M. bovis, were destroyed by treatment with 10% solution of the detergent for more than 60 minutes. M. bovis was relatively sensitive to the detergent, and was killed by 5% solution for 30 min. and 10% for 15 min., but M. avium was very resistant, and was not killed by 10% solution for 240 min.
2) By treatment with the detergent at 50°C, the antimycobacterial activities of the detergent were highly increased. All mycobacteria were destroyed by 1% solution for 180 min, and by 10% for less than 30 min at 50°C
In the comparison of the antibacterial activities against mycobacteria and other bacteria, it shows that mycobacteria are generally more resistant to the detergent, Hygien 51.
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